Edoardo Morandi, Ilaria Manfredonia, Lisa M. Simon, Francesca Anselmi, Martijn J. van Hemert, Salvatore Oliviero and Danny Incarnato
RNA structure heterogeneity represents the major challenge when querying RNA structures with chemical probing. We here introduce DRACO, an algorithm
for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome by DMS-MaPseq and DRACO
identifies multiple regions folding into two mutually-exclusive conformations, including a conserved structural switch in the 3′ UTR. Our work opens
the way to dissecting the heterogeneity of the RNA structurome.
RC: RNA Count (per base mutation count and coverage)
MM: Mutation Map (map of mutations per read, input for DRACO)
JSON: DRACO′s raw output
XML: Normalized reactivities
CM: Covariance model
Stockholm: Alignment of structurally-related CoVs
cspA 5′ UTR (RC, MM, JSON and XML files) | Download | |
V. vulnificus add riboswitch (RC, MM, JSON and XML files) | Download | |
SARS-CoV-2 (RC, JSON and XML files) | Download | |
SARS-CoV-2 3'-UTR conformations (CM and Stockholm files) | Download | |
SARS-CoV-2, replicate #1 (MM file) | Download | |
SARS-CoV-2, replicate #2 (MM file) | Download | |
DRACO algorithm (source code) | GitHub |